Coding

Part:BBa_K1334017:Design

Designed by: Qiushuang Wu   Group: iGEM14_WHU-China   (2014-09-27)


PADH from Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 880
    Illegal AgeI site found at 211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 123


Design Notes

The original sequence contains a Not1 site,by point mutation,we removed the site


Source

Using the genomic DNA of P. putida as the template, the coding regions of the PADH gene (padh) was amplified by PCR. The PCR products were then subcloned into PSB1C3

References